Comparison of semen quality in young and mature Holstein bulls measured by light microscopy and flow cytometry.

Improvement of Semen Quality in Holstein Bulls during Heat Stress by Dietary Supplementation of Omega-3 Fatty Acids

Environmental and Genetic Factors Affecting on Semen Quality in Iranian Holstein Bulls

The objective of this study was to evaluate the importance of environmental and genetic factors affecting semen quality; sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh motile sperm in each milt ejac...

Effects of egg yolk-citrate and milk extenders on chromatin structure and viability of cryopreserved bull sperm.

Semen from four Holstein bulls was evaluated to compare effects of four extender treatments on postthaw semen quality. Extender fractions A and B, either heated whole milk or 20% egg yolk-citrate, were combined to yield the extender treatments 1) milk and milk, 2) milk and egg yolk-citrate, 3) egg yolk-citrate and milk, and 4) egg yolk-citrate and egg yolk-citrate. Semen was evaluated at thawin...

P-95: Flow Cytometry Analysis of Bovine Semen:A Qualitative Study

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...

New Flow Cytometric Methods for Evaluating Bull Semen

Bull fertility and semen quality are of paramount importance to the cattle industry. Fluorescent biomarkers measured by flow cytometry can aid in the detection of sub-fertile bulls as they can identify abnormal spermatozoa that may not be properly identified as inferior with standard methods of subjective semen analysis. This paper introduces the basics of flow cytometry, discusses fluorescent ...